다양한 탄수화물 조건하에서 배양된 Streptococcus mutans의 자당의존성 세균부착과 관련된 우식유발능 차이
The differential sucrose-dependent cariogenic traits of Streptococcus mutans in the presence of dietary carbohydrates
이영은, 이형숙, 송근배,
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이영은 ( Lee Young-Eun ) - 대구보건대학 치위생과
이형숙 ( Lee Hyung-Suk ) - 대구보건대학 치위생과
송근배 ( Song Keun-Bae ) - 경북대학교 치의학전문대학원 예방치과학교실
KMID : 0355420110350030251
Abstract
Objectives: The aim of this study was to investigate the differential sucrose-dependent cariogenic traits of Streptococcus mutans in the presence of dietary carbohydrates.
Methods: S. mutans KCTC3065 were cultured in TYE medium containing 0.5% glucose at 37oC under 10% CO2 for 24 hours. The strain was divided into the following 5 groups in terms of the culture medium that was used: (1) 0.5% glucose containing basal culture TYE medium (BCM), (2) BCM plus 0.5% sucrose, (3) BCM plus 0.5% sucrose and fructose, (4) BCM plus 0.5% sucrose and maltose, (5) BCM plus 0.5% sucrose, fructose and maltose. After the cells were collected, growth, acid production synthesis of extracellular polysarcharides and related gene expression were evaluated.
Results: The growth and acid production of S. mutans in the presence of sucrose and fructose were delayed and decreased, but not in the presence of sucrose and maltose (p＜0.05). The expression level of virulence-related genes associated with gtfB and gtfD were significantly difference under all conditions (p＜0.05). Moreover, there were more extracellular polysaccharides due to gtfB and gtfD expression in the presence of sucrose, fructose, and maltose.
Conclusions: These results indicate that the growth and acid production of S. mutans were affected by the presence of sucrose, fructose and maltose, which also effectively increased extracellular polysarccharides synthesis by gtfB and gtfD expression. Thus, sucrose-dependent cariogenic traits of S. mutans can be increased.
키워드
자당의존성 우식유발능; 탄수화물
carbohydrates; Streptococcus mutans; sucrose-dependent cariogenic traits
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